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microscope nikon eclipse 90i epifluorescence microscope  (Nikon)


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    Nikon microscope nikon eclipse 90i epifluorescence microscope
    Microscope Nikon Eclipse 90i Epifluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microscope nikon eclipse 90i epifluorescence microscope/product/Nikon
    Average 90 stars, based on 1 article reviews
    microscope nikon eclipse 90i epifluorescence microscope - by Bioz Stars, 2026-05
    90/100 stars

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    Adult salivary gland cellular architecture of the cat flea ( C. felis ). (A) Fluorescent staining of female salivary glands dissected 3-days post-feeding. The tissues were fixed with Bouin's solution and permeabilized using 0.1 % Triton X-100 in PBS. Nuclei were labeled with DAPI (blue signal in merged images), and salivary glands were counterstained with Evans blue (red fluorescent signal in merged images). Scale bar represents 100 μm (upper row) and 50 μm (lower row); (B) Fluorescent staining of female salivary glands dissected 7-days post-feeding. The samples were fixed with 4 % paraformaldehyde in cytoskeleton-stabilizing PHEM buffer and permeabilized using 0.1 % Triton X-100 in PBS. Actin filaments were labeled with conjugated phalloidin (purple signal), and nuclei were stained with DAPI (blue signal). Salivary glands were treated with a Vector TrueVIEW autofluorescence quenching kit. Scale bar represents 100 μm; (C) Comparative images of female salivary glands 1- and 14-days post-feeding (dpf), and differences in salivary gland structure between sexes. The preparations were fixed with Bouin's solution and permeabilized with 0.1 % Triton X-100 in PBS. Gland and duct nuclei were visualized with DAPI (blue signal). Scale bar represents 100 μm. The terminology of the flea's internal anatomy is according to . Abbreviations: BF, bright-field microscopy; CD, striated cuticle of duct; EC, epithelial (secretory) cell; L, lumen; N, nucleus; SD, salivary duct; SG, salivary glands. Micrographs on panel A were taken using a Nikon Eclipse <t>90i</t> <t>microscope,</t> and images on panels B and C were captured using an LSM 980 Airy Scan II confocal microscope. Created with BioRender.com .
    Eclipse 90i Epifluorescent Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    epifluorescence microscope nikon eclipse 90i  (Nikon)
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    Nikon epifluorescence microscope nikon eclipse 90i
    Adult salivary gland cellular architecture of the cat flea ( C. felis ). (A) Fluorescent staining of female salivary glands dissected 3-days post-feeding. The tissues were fixed with Bouin's solution and permeabilized using 0.1 % Triton X-100 in PBS. Nuclei were labeled with DAPI (blue signal in merged images), and salivary glands were counterstained with Evans blue (red fluorescent signal in merged images). Scale bar represents 100 μm (upper row) and 50 μm (lower row); (B) Fluorescent staining of female salivary glands dissected 7-days post-feeding. The samples were fixed with 4 % paraformaldehyde in cytoskeleton-stabilizing PHEM buffer and permeabilized using 0.1 % Triton X-100 in PBS. Actin filaments were labeled with conjugated phalloidin (purple signal), and nuclei were stained with DAPI (blue signal). Salivary glands were treated with a Vector TrueVIEW autofluorescence quenching kit. Scale bar represents 100 μm; (C) Comparative images of female salivary glands 1- and 14-days post-feeding (dpf), and differences in salivary gland structure between sexes. The preparations were fixed with Bouin's solution and permeabilized with 0.1 % Triton X-100 in PBS. Gland and duct nuclei were visualized with DAPI (blue signal). Scale bar represents 100 μm. The terminology of the flea's internal anatomy is according to . Abbreviations: BF, bright-field microscopy; CD, striated cuticle of duct; EC, epithelial (secretory) cell; L, lumen; N, nucleus; SD, salivary duct; SG, salivary glands. Micrographs on panel A were taken using a Nikon Eclipse <t>90i</t> <t>microscope,</t> and images on panels B and C were captured using an LSM 980 Airy Scan II confocal microscope. Created with BioRender.com .
    Epifluorescence Microscope Nikon Eclipse 90i, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/epifluorescence microscope nikon eclipse 90i/product/Nikon
    Average 90 stars, based on 1 article reviews
    epifluorescence microscope nikon eclipse 90i - by Bioz Stars, 2026-05
    90/100 stars
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    Adult salivary gland cellular architecture of the cat flea ( C. felis ). (A) Fluorescent staining of female salivary glands dissected 3-days post-feeding. The tissues were fixed with Bouin's solution and permeabilized using 0.1 % Triton X-100 in PBS. Nuclei were labeled with DAPI (blue signal in merged images), and salivary glands were counterstained with Evans blue (red fluorescent signal in merged images). Scale bar represents 100 μm (upper row) and 50 μm (lower row); (B) Fluorescent staining of female salivary glands dissected 7-days post-feeding. The samples were fixed with 4 % paraformaldehyde in cytoskeleton-stabilizing PHEM buffer and permeabilized using 0.1 % Triton X-100 in PBS. Actin filaments were labeled with conjugated phalloidin (purple signal), and nuclei were stained with DAPI (blue signal). Salivary glands were treated with a Vector TrueVIEW autofluorescence quenching kit. Scale bar represents 100 μm; (C) Comparative images of female salivary glands 1- and 14-days post-feeding (dpf), and differences in salivary gland structure between sexes. The preparations were fixed with Bouin's solution and permeabilized with 0.1 % Triton X-100 in PBS. Gland and duct nuclei were visualized with DAPI (blue signal). Scale bar represents 100 μm. The terminology of the flea's internal anatomy is according to . Abbreviations: BF, bright-field microscopy; CD, striated cuticle of duct; EC, epithelial (secretory) cell; L, lumen; N, nucleus; SD, salivary duct; SG, salivary glands. Micrographs on panel A were taken using a Nikon Eclipse 90i microscope, and images on panels B and C were captured using an LSM 980 Airy Scan II confocal microscope. Created with BioRender.com .

    Journal: Current Research in Insect Science

    Article Title: Salivary glands of the cat flea, Ctenocephalides felis : Dissection and microscopy guide

    doi: 10.1016/j.cris.2024.100080

    Figure Lengend Snippet: Adult salivary gland cellular architecture of the cat flea ( C. felis ). (A) Fluorescent staining of female salivary glands dissected 3-days post-feeding. The tissues were fixed with Bouin's solution and permeabilized using 0.1 % Triton X-100 in PBS. Nuclei were labeled with DAPI (blue signal in merged images), and salivary glands were counterstained with Evans blue (red fluorescent signal in merged images). Scale bar represents 100 μm (upper row) and 50 μm (lower row); (B) Fluorescent staining of female salivary glands dissected 7-days post-feeding. The samples were fixed with 4 % paraformaldehyde in cytoskeleton-stabilizing PHEM buffer and permeabilized using 0.1 % Triton X-100 in PBS. Actin filaments were labeled with conjugated phalloidin (purple signal), and nuclei were stained with DAPI (blue signal). Salivary glands were treated with a Vector TrueVIEW autofluorescence quenching kit. Scale bar represents 100 μm; (C) Comparative images of female salivary glands 1- and 14-days post-feeding (dpf), and differences in salivary gland structure between sexes. The preparations were fixed with Bouin's solution and permeabilized with 0.1 % Triton X-100 in PBS. Gland and duct nuclei were visualized with DAPI (blue signal). Scale bar represents 100 μm. The terminology of the flea's internal anatomy is according to . Abbreviations: BF, bright-field microscopy; CD, striated cuticle of duct; EC, epithelial (secretory) cell; L, lumen; N, nucleus; SD, salivary duct; SG, salivary glands. Micrographs on panel A were taken using a Nikon Eclipse 90i microscope, and images on panels B and C were captured using an LSM 980 Airy Scan II confocal microscope. Created with BioRender.com .

    Article Snippet: The preparations were observed using a Zeiss LSM 980 Airy Scan II confocal microscope or Nikon Eclipse 90i epifluorescent microscope.

    Techniques: Staining, Labeling, Plasmid Preparation, Microscopy